Ultra Fast: <10 min operation time
High Sensitivity: More sensitive than silver staining
Mass Compatible: No bound component and ideal for mass spectrometry
Reversible: Easy destaining and can be further stained by other staining methods

In some circumstance, the pH value and wetness of 2D gels are greatly changed during long time of electrophoresis. It is highly recommended to turning your 2D gel conditions by submerge it in SDS electro-phoresis buffer (Tris/glycine buffer) for 5-10 minutes before adding Solution I. It is not required to wash the tuned gel before adding Solution I.

50ml and 100ml of Solution I/II are sufficient to stain 16x18cm and 24x20 cm 2D gels respectively.

Because the 2D gel is often thicker, it's recommended to extend incubation of Solution I to 10-15 minutes for better staining results. It's recommended to cut the desired protein spots for the stained gel right after the staining.

 

 

 

 

 

Vispro 5 Minutes Prostain Stain Kit	Silver Stain

VisPRO™ 5 Minutes Protein Stain perfectly matches to current proteomic research. Generally, more protein spots can be detected in 2-DE gels by this tobust staining method, compared to silver and Sypro Ruby staining. Moreover, theunfixing nature have it very ideal for subsequent analysis by mass spectrometry. Proteins develop by VisPRO? 5 Minutes Protein Stain usually have higher rate of good annotation.

Vispro 5 Minutes Prostain Stain Kit	Sypro Ruby stain	Silver Stain	CBR Stain

VisPRO™ 5 minutes Protein Stain Kit can detect proteins as little as 1 ng, comparable to silver and Sypro Ruby stain, and much more sensitive than Coomassie Brilliant blue stain. Protein from microgram can be visualized with good staining dynamic ranges. Unlike other staining methods requiring the interaction between dye molecules and proteins that generates the staining preference between proteins species, the negative staining nature makes VisPRO? 5min Protein Stain Kit visualizes all proteins.

VisPRO- 5 minutes Protein Stain reaction can be completely erased. Stained gels can be re-stained by other staining methods. Besides proteins in stained gels can be further eluted or electroblotted onto PVDF or nitrocellulose membranes. In additions, proteins on stained gel can be analyzed by mass spectrometry.

The MALDI- TOF spectrum of spots from zinc reverse staining

The Vispro Protein Saining spot of phosphorylase b was cut and subjected to mass sample preparation. The observed ionized fragments of rabbit phosphorylase b were annotated.