 |
 |
| ¡@ |
 |
Product
> Reagent > Prostain System |
|
| ¡@ |
|
| ¡@ |
|
¡@Key
Feature |
| ¡@ |
¡@ |
| Ultra
Fast: |
¡@<10 min operation
time |
High Sensitivity: |
¡@More sensitive than silver staining |
Mass
Compatible: |
¡@No bound component
and ideal for mass spectrometry |
Reversible: |
¡@Easy destaining and can be further
stained by other staining methods |
|
|
| ¡@ |
|
¡@Important
notification for staining 2D gels!! |
| |
|
In some circumstance,
the pH value and wetness of 2D gels
are greatly changed during long time
of electrophoresis. It is highly recommended
to turning your 2D gel conditions by
submerge it in SDS electro-phoresis
buffer (Tris/glycine buffer) for 5-10
minutes before adding Solution I. It
is not required to wash the tuned gel
before adding Solution I.
50ml and 100ml of Solution I/II are
sufficient to stain 16x18cm and 24x20
cm 2D gels respectively.
Because the 2D gel is often thicker,
it's recommended to extend incubation
of Solution I to 10-15 minutes for better
staining results. It's recommended to
cut the desired protein spots for the
stained gel right after the staining.
|
|
|
| ¡@ |
|
¡@Application
in Current Proteomics |
| ¡@ |
| |
|
Vispro 5
Minutes Prostain Stain Kit |
Silver Stain |
|
| ¡@ |
| VisPRO™ 5 Minutes Protein Stain
perfectly matches to current proteomic
research. Generally, more protein spots
can be detected in 2-DE gels by this
tobust staining method, compared to
silver and Sypro Ruby staining. Moreover,
theunfixing nature have it very ideal
for subsequent analysis by mass spectrometry.
Proteins develop by VisPRO? 5 Minutes
Protein Stain usually have higher rate
of good annotation. |
|
|
| ¡@ |
|
¡@Excellent
Sensitivity |
| ¡@ |
| |
|
Vispro 5
Minutes Prostain Stain Kit |
Sypro Ruby
stain |
|
|
Silver Stain |
CBR Stain |
|
| ¡@ |
| VisPRO™ 5 minutes Protein Stain
Kit can detect proteins as little as
1 ng, comparable to silver and Sypro
Ruby stain, and much more sensitive
than Coomassie Brilliant blue stain.
Protein from microgram can be visualized
with good staining dynamic ranges. Unlike
other staining methods requiring the
interaction between dye molecules and
proteins that generates the staining
preference between proteins species,
the negative staining nature makes VisPRO?
5min Protein Stain Kit visualizes all
proteins. |
| ¡@ |
|
|
| ¡@ |
|
¡@Full
Compatible with Downstream Applications |
| ¡@ |
| VisPRO- 5 minutes Protein Stain reaction
can be completely erased. Stained gels
can be re-stained by other staining
methods. Besides proteins in stained
gels can be further eluted or electroblotted
onto PVDF or nitrocellulose membranes.
In additions, proteins on stained gel
can be analyzed by mass spectrometry. |
| |
The MALDI-
TOF spectrum of spots from zinc
reverse staining. |
|
| The Vispro Protein Saining spot of
phosphorylase b was cut and subjected
to mass sample preparation. The observed
ionized fragments of rabbit phosphorylase
b were annotated. |
|
|
| ¡@ |
|
¡@Comparison
of Various kinds of Decoration Methods |
| ¡@ |
Method |
VisPRO™
5 minutes
Protein Stain kit |
Sypro
Ruby |
Silver
Stain |
CBR
Stain |
Image
Equipment |
Visible
light |
bioluminescence |
Visible
light |
Visible
light |
Stimulate
and
Toxicity Chemical |
no |
Acetic
acid,
Methanol |
Acetic
acid,
Silver Nitrate,
Glutaraldehyde |
Acetic acid,
Methanol |
Sensitivity |
1
ng |
1
ng |
1
ng |
50 ng |
Linear
range |
1-2000
ng |
1-1000
ng |
1-100ng |
50-1000
ng |
Downstream
Exp |
Yes |
Yes
|
No
(Protein Cross link) |
Yes, but
limited |
Time
Consuming |
5-10
min |
18
Hrs |
4-20
Hrs |
1-8
Hrs |
|
|
|
| ¡@ |
| ¡@ |
|
¡@Ordering
Information |
| ¡@ |
Cat
No |
Product
Name |
Specification |
VP-PS001 |
Prostain
system |
VisPRO™
5 Minutes Protein Stain Kit
x 5
LIAS Xlite 30R Nonlinear Phoretix
2D Advanced |
VP-PS101 |
VisPRO™
5 Minutes Protein Stain Kit |
Sensitizing
solution 250ml x 2
Developing solution 250ml x
2 |
AG-LA030 |
LIAS
Xlite 30R |
Avegene
Stanard
|
NL-PH2DA |
Nonlinear
Phoretix 2D Advance |
1xCD Professional
2D gel analysis software
|
|
|
|
| ¡@ |
| ¡@ |
| ¡@ |
