Option 1:
We suggest using a flatbed scanner with transparency unit to document the reverse stained gel images. Whiling scanning, turn on the transparency mode and save the image into 8 or 16 bit grayscale TIF file. For optimal image quality, stretching the level of image to maximal (0-255) is suggested before saving the files.
Option 2:
Gels can also be documented by flatbed scanner without transparency unit. However, a black background should be placed behind the gel while scanning.

The Dynamic range of the image is the number of grey levels to which the image is digitized in the camera. The Dynamic Range is directly related to the number of bits of the camera. The more bits the camera then the greater the number of grey levels or grey scale it has. The Xlite has a 12-bit A/D (analog/digital) converter and the system provides a maximum linear dynamic range of 3.6 orders of magnitude. Xlite can easily detect very bright bands and very faint bands within the same image.

Each camera is different.

Check that the correct filter is selected for the chosen application. For UV applications use the UV filter.

Check the aperture on your lens is fully open. In addition, using the integration control to increase the camera exposure - this will make the image brighter. To get the correct aperture/integration simply and quickly while avoiding over exposure, use the Auto-Exposure and over-exposure function then you can capture the correct integration time for the set aperture as well as assessing the saturation of the image during the process.

Saturation occurs when an image is over exposed. This means that part or whole image exceeds the dynamic range of the camera. When images saturated they are unsuitable for quantification as it is impossible to measure genuine differences in intensity. Xlite series have a live Saturation Detection function. When activated over-exposed a warning will shown on the display panel. To avoid these, manually decrease the integration time until warning disappeared. Another method is to use the Auto-Exposure function which gives the brightest image possible without saturation.

Any printer can be used to print Xlite images as long as it has Windows drivers. The most common are laser, inject, thermal or dye sublimation printers.

No, they don’t. Xlite series are standalone systems. You can take any pictures by clicking a button on control panel.

If you want to control your Xlite system by your computer, the Xlite series are compatible with WINDOW2000, XP. It is important to remember the Xlite series require IEEE 1394（FIREWIRE）communication and “Xview” software（purchasing from Avegene）

Absolutely, the images can be used for all the popular journals. Images in TIFF file format are usually accepted.

Option 1:
We suggest using a flatbed scanner with transparency unit to document the reverse stained gel images. Whiling scanning, turn on the transparency mode and save the image into 8 or 16 bit grayscale TIF file. For optimal image quality, stretching the level of image to maximal (0-255) is suggested before saving the files.
Option 2:
Gels can also be documented by flatbed scanner without transparency unit. However, a black background should be placed behind the gel while scanning.

Yes. In fact, only with a silver stain must you first destain the gel. The low pH of the Coomassie staining solution acts as a destainer.

We recommend using the Gel Lighting Plate (Product code: GL1001) to visualize the gel. It has been proved that gel lighting plate is excellent for direct observing the reverse stained gels. (Reference: Ching-Yu Lin, Hui-Ming Huang, and Han-Min Chen)
Use of backlit light plate to enhance visualization of imidazole-zinc reverse stained gels: BioTechniques 41:560-564 (November 2006).

The protein band without any color, it is transparent.
The color is come from the background.
Please check the stained gel by black background or using our gel lighting plate.

Please check the stained gel by black background or using our gel lighting plate.

No, we do not suggest that because the components of solution I and II will change if you reuse.

We suggest customer have fixed gel first because the protein will diffuse if without fixing gel.

Definitely! However, you should wash such gels by distilled water for 10 minutes, and equilibrate in SDS electrophoresis buffer for another 10 minutes. The washed gels can be subsequently developed by VisPRO 5 minutes Protein Stain Kit following the standard staining protocol.

Option 1:
Destaining by SDS electrophoresis buffer (regular Tris/Glycine buffer with SDS) for 10 minutes. This destaining method is used where restaining by VisPro 5 minutes Protein Stain or membrane transferring is required. Option 2:
Destaining by 10% acetic acid for 5-30 minutes. This destaining method is used where fixing of the protein is required, such as preparing the samples for mass spectrometry (MS) or restaining by other gel staining methods, such as silver stain, CBB stain or Sypro Ruby stain.

It might happen if pouring Solution II directly onto gel surfaces, or agitating is not vigorous enough while development. You can destain the gel and then restain it according to the manual’s instructions.